lytG [2019-09-23 09:44:09]

peptidoglycan N-acetylglucosaminidase

locus: BSU_31120

pI: 8.28

mw: 31.75 kDa

protein length: 282 aa

gene length: 849 bp

function: cell wall turnover

product: peptidoglycan N-acetylglucosaminidase

essential: no

synonyms: yubE

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.5|N-acetyl-β-D-glucosaminidases]

Gene

Coordinates: 3,190,834 → 3,191,682

The protein

Catalyzed reaction/ biological activity

• hydrolysis of the glycosidic bond between N-acetyl-β-d-glucosamine residues and adjacent monosaccharides [Pubmed|18266855]

Protein family

• glycosyl hydrolase 73 family (with [protein|29219315D31BA4ADE41687EFF17FE41D6C23D157|LytD], according to UniProt)

[SW|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|AF0BC3D534BC2CCCAAB53B286CF4286E58A8A338|lytG]-[gene|E1549CE5B1648EBD1E23890D97F0B545A9EC10F6|yubF]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A027 (yubE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/27 NBRP B. subtilis, Japan]
• BKE31120 (Δ[gene|AF0BC3D534BC2CCCAAB53B286CF4286E58A8A338|lytG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAAACCTTCCTTCGAA, downstream forward: _UP4_TAAGCCCTTTTTACTGGTAT
• BKK31120 (Δ[gene|AF0BC3D534BC2CCCAAB53B286CF4286E58A8A338|lytG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAAACCTTCCTTCGAA, downstream forward: _UP4_TAAGCCCTTTTTACTGGTAT

References

Reviews

• 18266855

Original publications

• 12525152,17427287,28448118